Alkaline peptone water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. This medium may also be used for the enrichment of Vibrio spp. from food and water samples.
First developed by Shread, Donovan and Lee as an enrichment broth for the growth of Aeromonas spp., Cruickshank showed that with a higher pH the medium can be used for the enrichment of Vibrio spp.
The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance. The high pH of the medium inhibits most enteric organisms for at least 24 hours.
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