Vitamin B12 (Cyanocobalamin) Test Kit

【Principles】

Vitamin B12 (Cyanocobalamin) Test Kit used microbiology method to detect the volume of VB12 in food matrices. VB12 supplies the growth of Lactobacillus delbrueckiikit. Its growth rate associated with the volume of VB12 within the sample. Take the VB12 medium (Lactobacillus delbrueckiikit added), samples or Standards and add to the microwell. Incubate at 37℃ and avoid light exposure. Lactobacillus delbrueckiikit will start growing until VB12 are consumed entirely. It takes 44-48 hours to complete incubation. Use microplate reader to read the sample turbidity with 610-630nm or 540-550nm filter. The volume of VB12 in sample can be determined by comparing the sample turbidity with the Standards.

【Provided Materials and Reagent】

           Component                     Qty

• Microwell plate              96T

• Cover film                          3

• Plate rack                           1

• Reagent reservoir            3

• Package Insert                  1

• Testing Report                  1

• Sterile water                     3 bottles

• VB12 Medium                  3 bottles

• VB12 Standards               3 bottles

• VB12 Assay Strain           3 bottles